Development of Specific Molecular and Phenotypic Marker-Based Haploid Inducers in Rice

Doubled haploid (DH) technology is an efficient strategy for producing completely homozygous lines breeding programs. Mutations in the MATRILINEAL (MTL) phospholipase trigger intraspecific induction cereals. Although vivo system based on OsMTL-edited plants has been established rice (Oryza sativa), DH still limited by other factors, such as identification, which one of essential steps required technology. In study, we addressed this technical challenge integrating specific molecular and phenotypic markers into inducers. We first generated large fragment insertion or deletion mutations within OsMTL gene designed a pair primers flanking mutational sites to be used universal between wild-type Osmtl plants. Next, screened hairy leaf single dominant trait integrated it marker-based inducers using cross self-cross method. When crossing cytoplasmic male sterile with these inducers, utilized InDel marker identify putative haploids (or double haploids). These were further confirmed through ploidy analysis, demonstrating high efficiency identification markers. The rate (HIR) developed ranged from 3.7% 12.5%. have achieved successful integration distinct Our advanced significantly enhanced accuracy thereby expediting adoption breeding.